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multiscribe reverse transcriptase kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher multiscribe reverse transcriptase kit
    Multiscribe Reverse Transcriptase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiscribe+reverse+transcriptase+kit/multiscribe+reverse+transcriptase+kit/pmc12260516-107-13-17
    Average 90 stars, based on 1 article reviews
    multiscribe reverse transcriptase kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Lysis:

    Article Title: Dietary intake and glutamine-serine metabolism control pathologic vascular stiffness.
    Article Snippet: In brief Rachedi et al. identify the precise metabolic requirements for pathogenic vascular fibroblast activation, using pulmonary arterial hypertension as an example.. Inflammation, hypoxia, and mechanical stress converge on YAP and TAZ activation.. YAP and TAZ control glutamine and serine catabolism to generate proline and glycine for collagen biosynthesis.

    Collagen Assay:

    Article Title: Dietary intake and glutamine-serine metabolism control pathologic vascular stiffness.
    Article Snippet: In brief Rachedi et al. identify the precise metabolic requirements for pathogenic vascular fibroblast activation, using pulmonary arterial hypertension as an example.. Inflammation, hypoxia, and mechanical stress converge on YAP and TAZ activation.. YAP and TAZ control glutamine and serine catabolism to generate proline and glycine for collagen biosynthesis.

    Reverse Transcription:

    Article Title: Dietary intake and glutamine-serine metabolism control pathologic vascular stiffness.
    Article Snippet: In brief Rachedi et al. identify the precise metabolic requirements for pathogenic vascular fibroblast activation, using pulmonary arterial hypertension as an example.. Inflammation, hypoxia, and mechanical stress converge on YAP and TAZ activation.. YAP and TAZ control glutamine and serine catabolism to generate proline and glycine for collagen biosynthesis.

    Article Title: Adipsin-dependent adipocyte maturation induces cancer cell invasion in breast cancer.
    Article Snippet: .. First-strand cDNA extracted from samples was synthesized from total RNA (1 μg) using a MultiScribe Reverse Transcriptase Kit (Thermo Fisher Scientific). ..

    Article Title: Activation of Peroxisome Proliferator-Activated Receptor-β/δ (PPARβ/δ) in Keratinocytes by Endogenous Fatty Acids
    Article Snippet: .. The cDNA was generated using 2.5 μg total RNA with the MultiScribe Reverse Transcriptase kit (Applied Biosystems, Foster City, CA, USA). .. Primers were designed for real-time PCR using the Primer Express software (Version 3.0, Applied Biosystems, Foster City, CA, USA).

    Article Title: Interaction between a J-domain co-chaperone and a specific Argonaute protein contributes to microRNA function in animals
    Article Snippet: .. For mRNA quantification, reverse transcription was performed using 400 ng of RNA with Multiscribe Reverse Transcriptase Kit (Applied Biosystems) (25°C for 10 min, 37°C for 120 min and 85°C for 5 min) followed by RNase H treatment (1 ul per RT) at 37°C for 30 min. SYBR Green RT-qPCR was performed using 2 ul of cDNA with the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) on a QuantStudio 3 machine with regular settings (95°C for 10 min and 40 cycles at 95°C for 15 s and 60°C for 60 s). ..

    Article Title: Investigations Towards Tryptophan Uptake and Transport Across an In Vitro Model of the Oral Mucosa Epithelium
    Article Snippet: RNA was isolated using the NucleoSpin RNA Kit (Machery Nagel, 740961) according to the manufacturer’s instruction and eluted with 40 μL nuclease-free water (Invitrogen, AM9937). .. For cDNA synthesis 1000 ng RNA was applied using the Multiscribe Reverse Transcriptase Kit (Applied Biosystem Thermo Scientific, 4311235). ..

    Article Title: Targeting Intracellular Innate RNA-Sensing Systems Overcomes Resistance to CAR T-cell Therapy in Solid Tumors
    Article Snippet: Total RNA was extracted from sorted tumor cell pellets using the innuPREP RNA mini kit 2.0 (Analytik Jena). .. RNA concentrations were measured using a NanoDrop spectrophotometer. cDNA was reverse-transcribed using the MultiScribe Reverse Transcriptase kit (Thermo Fisher Scientific). .. Using PowerUp SYBR Green Master Mix (Thermo Fisher Scientific), 2 ng of cDNA was analyzed by qPCR in a QuantStudio 5 Real Time PCR System.

    Article Title: Diet-Induced Obesity in the Rat Impairs Sphingolipid Metabolism in the Brain and This Metabolic Dysfunction Is Transmitted to the Offspring via Both the Maternal and the Paternal Lineage.
    Article Snippet: Obesity leads to a number of health problems, including learning and memory deficits that can be passed on to the offspring via a developmental programming process.. However, the mechanisms involved in the deleterious effects of obesity on cognition remain largely unknown.. This study aimed to assess the impact of obesity on the production of sphingolipids (ceramides and sphingomyelins) in the brain and its relationship with the learning deficits displayed by obese individuals.

    Article Title: Exon Skipping-based Subtyping of Colorectal Cancers.
    Article Snippet: BACKGROUND AND AIMS: The identification of colorectal cancer (CRC) molecular subtypes has prognostic and potentially diagnostic value for patients, yet reliable subtyping remains unavailable in the clinic.. The current consensus molecular subtype (CMS) classification in colorectal cancers is based on complex RNA expression patterns quantified at gene level.. The clinical application of these methods, however, is challenging due to high uncertainty of single sample classification and associated costs.

    Negative Control:

    Article Title: Dietary intake and glutamine-serine metabolism control pathologic vascular stiffness.
    Article Snippet: In brief Rachedi et al. identify the precise metabolic requirements for pathogenic vascular fibroblast activation, using pulmonary arterial hypertension as an example.. Inflammation, hypoxia, and mechanical stress converge on YAP and TAZ activation.. YAP and TAZ control glutamine and serine catabolism to generate proline and glycine for collagen biosynthesis.

    Recombinant:

    Article Title: Dietary intake and glutamine-serine metabolism control pathologic vascular stiffness.
    Article Snippet: In brief Rachedi et al. identify the precise metabolic requirements for pathogenic vascular fibroblast activation, using pulmonary arterial hypertension as an example.. Inflammation, hypoxia, and mechanical stress converge on YAP and TAZ activation.. YAP and TAZ control glutamine and serine catabolism to generate proline and glycine for collagen biosynthesis.

    Plasmid Preparation:

    Article Title: Dietary intake and glutamine-serine metabolism control pathologic vascular stiffness.
    Article Snippet: In brief Rachedi et al. identify the precise metabolic requirements for pathogenic vascular fibroblast activation, using pulmonary arterial hypertension as an example.. Inflammation, hypoxia, and mechanical stress converge on YAP and TAZ activation.. YAP and TAZ control glutamine and serine catabolism to generate proline and glycine for collagen biosynthesis.

    Software:

    Article Title: Dietary intake and glutamine-serine metabolism control pathologic vascular stiffness.
    Article Snippet: In brief Rachedi et al. identify the precise metabolic requirements for pathogenic vascular fibroblast activation, using pulmonary arterial hypertension as an example.. Inflammation, hypoxia, and mechanical stress converge on YAP and TAZ activation.. YAP and TAZ control glutamine and serine catabolism to generate proline and glycine for collagen biosynthesis.

    Synthesized:

    Article Title: Adipsin-dependent adipocyte maturation induces cancer cell invasion in breast cancer.
    Article Snippet: .. First-strand cDNA extracted from samples was synthesized from total RNA (1 μg) using a MultiScribe Reverse Transcriptase Kit (Thermo Fisher Scientific). ..

    Generated:

    Article Title: Activation of Peroxisome Proliferator-Activated Receptor-β/δ (PPARβ/δ) in Keratinocytes by Endogenous Fatty Acids
    Article Snippet: .. The cDNA was generated using 2.5 μg total RNA with the MultiScribe Reverse Transcriptase kit (Applied Biosystems, Foster City, CA, USA). .. Primers were designed for real-time PCR using the Primer Express software (Version 3.0, Applied Biosystems, Foster City, CA, USA).

    SYBR Green Assay:

    Article Title: Interaction between a J-domain co-chaperone and a specific Argonaute protein contributes to microRNA function in animals
    Article Snippet: .. For mRNA quantification, reverse transcription was performed using 400 ng of RNA with Multiscribe Reverse Transcriptase Kit (Applied Biosystems) (25°C for 10 min, 37°C for 120 min and 85°C for 5 min) followed by RNase H treatment (1 ul per RT) at 37°C for 30 min. SYBR Green RT-qPCR was performed using 2 ul of cDNA with the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) on a QuantStudio 3 machine with regular settings (95°C for 10 min and 40 cycles at 95°C for 15 s and 60°C for 60 s). ..

    Quantitative RT-PCR:

    Article Title: Interaction between a J-domain co-chaperone and a specific Argonaute protein contributes to microRNA function in animals
    Article Snippet: .. For mRNA quantification, reverse transcription was performed using 400 ng of RNA with Multiscribe Reverse Transcriptase Kit (Applied Biosystems) (25°C for 10 min, 37°C for 120 min and 85°C for 5 min) followed by RNase H treatment (1 ul per RT) at 37°C for 30 min. SYBR Green RT-qPCR was performed using 2 ul of cDNA with the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) on a QuantStudio 3 machine with regular settings (95°C for 10 min and 40 cycles at 95°C for 15 s and 60°C for 60 s). ..

    cDNA Synthesis:

    Article Title: Investigations Towards Tryptophan Uptake and Transport Across an In Vitro Model of the Oral Mucosa Epithelium
    Article Snippet: RNA was isolated using the NucleoSpin RNA Kit (Machery Nagel, 740961) according to the manufacturer’s instruction and eluted with 40 μL nuclease-free water (Invitrogen, AM9937). .. For cDNA synthesis 1000 ng RNA was applied using the Multiscribe Reverse Transcriptase Kit (Applied Biosystem Thermo Scientific, 4311235). ..

    Article Title: Exon Skipping-based Subtyping of Colorectal Cancers.
    Article Snippet: BACKGROUND AND AIMS: The identification of colorectal cancer (CRC) molecular subtypes has prognostic and potentially diagnostic value for patients, yet reliable subtyping remains unavailable in the clinic.. The current consensus molecular subtype (CMS) classification in colorectal cancers is based on complex RNA expression patterns quantified at gene level.. The clinical application of these methods, however, is challenging due to high uncertainty of single sample classification and associated costs.

    Spectrophotometry:

    Article Title: Targeting Intracellular Innate RNA-Sensing Systems Overcomes Resistance to CAR T-cell Therapy in Solid Tumors
    Article Snippet: Total RNA was extracted from sorted tumor cell pellets using the innuPREP RNA mini kit 2.0 (Analytik Jena). .. RNA concentrations were measured using a NanoDrop spectrophotometer. cDNA was reverse-transcribed using the MultiScribe Reverse Transcriptase kit (Thermo Fisher Scientific). .. Using PowerUp SYBR Green Master Mix (Thermo Fisher Scientific), 2 ng of cDNA was analyzed by qPCR in a QuantStudio 5 Real Time PCR System.

    Purification:

    Article Title: Diet-Induced Obesity in the Rat Impairs Sphingolipid Metabolism in the Brain and This Metabolic Dysfunction Is Transmitted to the Offspring via Both the Maternal and the Paternal Lineage.
    Article Snippet: Obesity leads to a number of health problems, including learning and memory deficits that can be passed on to the offspring via a developmental programming process.. However, the mechanisms involved in the deleterious effects of obesity on cognition remain largely unknown.. This study aimed to assess the impact of obesity on the production of sphingolipids (ceramides and sphingomyelins) in the brain and its relationship with the learning deficits displayed by obese individuals.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Exon Skipping-based Subtyping of Colorectal Cancers.
    Article Snippet: BACKGROUND AND AIMS: The identification of colorectal cancer (CRC) molecular subtypes has prognostic and potentially diagnostic value for patients, yet reliable subtyping remains unavailable in the clinic.. The current consensus molecular subtype (CMS) classification in colorectal cancers is based on complex RNA expression patterns quantified at gene level.. The clinical application of these methods, however, is challenging due to high uncertainty of single sample classification and associated costs.

    Biomarker Discovery:

    Article Title: Exon Skipping-based Subtyping of Colorectal Cancers.
    Article Snippet: BACKGROUND AND AIMS: The identification of colorectal cancer (CRC) molecular subtypes has prognostic and potentially diagnostic value for patients, yet reliable subtyping remains unavailable in the clinic.. The current consensus molecular subtype (CMS) classification in colorectal cancers is based on complex RNA expression patterns quantified at gene level.. The clinical application of these methods, however, is challenging due to high uncertainty of single sample classification and associated costs.



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